中药复方对抑郁模型小鼠学习记忆能力及脑内SDF-1的影响     

杜娜  陈倩  李洋  肖婧珊  姚梦姣  李亚 《环球赛鸽科技》2015,(3)

目的：探讨中药复方枳菊解郁汤对抑郁模型小鼠空间学习记忆能力的影响及其机制。方法：采用多种因素慢性轻度不可预见性应激方式建立抑郁模型小鼠,分别给予小鼠低、中、高剂量枳菊解郁汤处理。采用Morris水迷宫实验,检测小鼠的空间学习记忆能力的变化;通过免疫组织化学方法检测脑内基质细胞衍生因子(SDF-1)的表达。结果：与对照组相比,模型组小鼠的空间学习记忆能力明显下降(P<0.01),SDF-1在海马CA1,CA3,DG及前脑皮层的表达明显降低(P<0.05);与模型组相比,枳菊解郁汤中剂量组小鼠的空间学习记忆能力明显增高(P<0.05),SDF-1在海马CA1,CA3,DG及前脑皮层的表达明显增加(P<0.05)。结论：枳菊解郁汤的抗抑郁作用可能与SDF-1的表达上调有关。  相似文献   

内质网应激介导细胞凋亡在运动预防非酒精性脂肪肝形成中的作用     

李军汉  苏全生  孙君志  彭丽文  谢红苑 《北京体育大学学报》2015,38(10):58-62+108

摘要：目的：探讨运动对非酒精性脂肪肝（NAFLD）的预防作用及作用机理。方法： SD雄性大鼠随机分为对照组、模型组和运动组3 组。对照组给予普通饲料喂养,模型组予高脂饲料喂养,运动组在高脂饲料喂养的同时予运动干预,连续8周,直至实验结束。HE染色观察肝脏病理形态变化,TUNEL法测肝细胞凋亡,western blot检测CHOP、Caspase12和JNK蛋白表达,免疫组化检测CHOP、Caspase12和JNK蛋白阳性表达。结果：1）与对照组比较,模型组肝细胞脂肪性变明显,肝细胞凋亡指数升高,CHOP、Caspase12、JNK蛋白表达及阳性表达增加;2）与模型组比较,运动组肝细胞脂肪变性减轻,肝细胞凋亡指数减少,CHOP、Caspase12、JNK蛋白表达及阳性表达降低。结论：8周高脂饮食可成功复制NAFLD动物模型,内质网应激介导细胞凋亡参与NAFLD形成。运动对NAFLD形成具有较好的预防作用,其机制可能与运动可降低内质网应激介导细胞凋亡蛋白CHOP、JNK和Caspase12在肝细胞的表达,抑制肝细胞凋亡有关。  相似文献   

Evaluation of Oxidative Stress and hsCRP in Polycystic Ovarian Syndrome in a Tertiary Care Hospital     

N. Unni C. Sumithra  R. Lakshman Lakshmi  N. Leela Menon  K. N. Subhakumari  V. S. Sheejamol 《Indian journal of clinical biochemistry : IJCB》2015,30(2):161-166

PCOS is a heterogeneous endocrine disorder with diverse clinical presentation. Oxidative stress plays a key role in the pathophysiology of this disease. Serumhigh sensitive C-reactive protein (hsCRP), a marker of chronic low grade inflammation, is indicative of future development of cardiovascular disease. Our aim is to evaluate the oxidant status and hsCRP levels in PCOS. The study involved 61 cases and 61 controls in the age group of 18–40 years diagnosed with PCOS. Erythrocyte malondialdehyde (MDA), superoxide dismutase (SOD), serum hsCRP, gonadotrophins, thyroid stimulating hormone, prolactin, glycemic status and lipid profile were estimated. Erythrocyte MDA (p < 0.001), SOD (p = 0.007) and serum hsCRP (p < 0.001) were significantly elevated in PCOS patients than controls. Oxidative stress is present in women with PCOS along with elevated hsCRP.  相似文献   

Association of Aberrations in One Carbon Metabolism with Intimal Medial Thickening in Patients with Type 2 Diabetes Mellitus     

R. Dhananjayan  T. Malati  Y. Rupasree  Vijay Kumar Kutala 《Indian journal of clinical biochemistry : IJCB》2015,30(3):263-270

The present work was aimed to study the association of one carbon genetic variants, hyperhomocysteinemia and oxidative stress markers, i.e., serum nitrite, plasma malondialdehyde (MDA) and glutathione (GSH) on intimal medial thickening (IMT) in patients with type 2 diabetes mellitus (T2D). A total number of 76 subjects from ACS Medical College and Hospital, Chennai, India were included in the study, i.e., Group I (n = 42) of T2D and Group II (n = 34) of age- and sex matched healthy controls. The glycated haemoglobin was measured by ion-exchange resin method; plasma homocysteine by Enzyme Linked Immunosorbant Assay method; serum nitrite (nitric oxide, NO), plasma MDA and GSH by spectrophotometric methods; the IMT by high frequency ultrasound. The polymorphisms of one carbon genetic variants were genotyped using polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism methods. Results indicate that methyltetrahydrofolate homocysteine methyl transferase (MTR) A2756G allele was found to be protective in T2D and the other variants were not significantly associated with T2D. Glutamate carboxypeptidase II (GCP II) C1561T (r = 0.34; p = 0.05) and methylene tetrahydrofolate reductase (MTHFR) C677T (r = 0.35; 0.04) showed positive correlation with plasma homocysteine in T2D cases. In this study, MTR A2756G allele was found to be protective in T2D; GCP II C1561T and MTHFR C677T showed positive association with plasma homocysteine in T2D cases. Among all the genetic variants, MTR A2756G was found influence IMT. RFC 1 G80A and TYMS 5′-UTR 2R3R showed synergistically interact with MTR A2756G in influencing increase in IMT.  相似文献   

基于情绪与压力管理的大学心理危机干预     

付小姣 《湖北函授大学学报》2015,(12):76-77

大学生不能合理应对内外部应激因素时会产生心理失衡状态,即心理危机。心理危机常伴随如焦虑、苦闷、沮丧、烦躁、愤怒、行为冲动等情绪和行为问题,对大学生心理危机干预应着重培养其情绪与压力管理能力。高校应积极开展情绪训练、建立健全情绪与压力管理体系,预防并有效处理大学生心理危机,促进大学生身心和谐发展。  相似文献   

由M_w7.8昆仑山地震地表位错推断滑动位移     

米琦  李彦恒  申文豪  史保平 《中国科学院大学学报》2015,32(5):652-660

2001年MW7.8昆仑山口西地震形成约400 km的地震破裂带,主震断层为一长度350 km、呈东西走向且近直立的走滑断层.主震断层附近的同震滑动位错为研究断层内部的力学性质及几何形态提供了重要参考.本文根据Freund的III-型位错模型,以实地测量和卫星测量的地表错动数据为约束条件,求取沿主震断层走向断层内部各点的滑动位移分布以及破裂面沿断层深度方向的扩展宽度.计算结果表明:III-型位错模拟结果所得断层面滑动位移分布特征和由有限断层模型体波反演得到的结果有较高的吻合程度,且在深度方向上呈现强烈的不均匀性.由III-型位错模型给出的断层内部的破裂宽度小于20 km,其平均值为8.4 km.进一步的对比分析表明:地表同震位移量的大小同断层的破裂宽度在尺度上存在明确的比例关系,沿断层走滑方向各点的滑动位移随深度的变化特征具有自相似性的分形特征.结合III-型位错模拟和体波反演结果,基于Andrews方法计算断层面上静态应力变化的分布,其分布特征同样具有很高的吻合度.  相似文献   

AtHsfAla 对拟南芥幼苗在干旱中生理指标的影响     

谭越  何承忠  郭丽红 《昆明师范高等专科学校学报》2015,(3)

以过量表达 AtHsfAla 及 AtHsfAla 基因沉默的转基因植株为材料,通过控制土壤水分进行干旱处理,结果显示,过量表达型干旱处理后的存活率最大,而基因沉默型最小,表明 AtHsfA1a 能增强植物的抗旱性。为了从生理水平研究 AtHsfA1a 提高抗旱性的机制,研究发现过量表达型的叶片相对含水量和叶绿素含量最高,而沉默型最低。不同基因型植株的 H2 O2和 MDA 的含量正好相反,说明 AtHsfA1a 通过保护细胞膜而减小细胞所受氧化伤害来提高抗旱能力。  相似文献   

高校辅导员职业压力应对策略研究——基于积极心理学角度     

李晓璇 《惠州学院学报》2015,(2):119-122

辅导员是高校中不可或缺的角色,是学生接触最多的一线管理者,他们的职业压力直接影响着学生管理工作的实际成效。本文分析了辅导员产生职业压力的内外部原因及其职业压力造成的负面影响,从积极心理学的角度,提出了塑造积极人格、构建积极的组织环境、提升辅导员的成就感和个人地位以及个人的工作技能等应对职业压力的策略。  相似文献   

Calculating method of contact stress for non-circular gears     

LI Ji-qiang  LIU Zhong-ming  YAN Shi-dang 《重庆大学学报(英文版)》2015,14(1):19-24

According to Hertz theory, the difference of contact stress for non-circular gears and equivalent gears is compared in the paper, a calculating method of contact stress for non-circular gears by using equivalent gears is researched, and computing formulas of power and rotation speed for equivalent gears are deduced. A numerical simulation of contact stress for non-circular gears has also been conducted based on the finite element method. By the comparison of fitting curves, the feasibility of using equivalent gears instead of non-circular gears to calculate the contact stress is testified.  相似文献   

盐胁迫对大豆POD、SOD和CAT同工酶的影响     

何庆元  向仕华  吴萍  王松华  李正鹏  祝嫦巍  张晓红 《安徽科技学院学报》2015,(3):10-13

采用1/2浓度Hoagland营养液培育法中不同浓度(0.000、0.050、0.075、0.100和0.125 mol/L)的Na Cl溶液处理V2期苗期大豆,待大豆生长至V3期,分析不同浓度盐胁迫对大豆生长以及POD、SOD和CAT同工酶的影响。结果表明:Na Cl浓度越高,大豆生长受到的抑制越大,而POD、SOD和CAT的活性在Na Cl浓度在0.050 mol/L有一定的增加,但增加到0.075 mol/L后,酶的活性降低。在0.075和0.100mol/L的Na Cl浓度诱导出了新的POD同工酶谱带,而0.125 mol/L Na Cl使SOD同工酶的2条谱带消失。  相似文献